Applications

Many possibilities exist for flow cytometric analysis or individual cell sorting, including, but not limited to:

  • Immunofluorescent measurements (surface and intracellular markers, single and multi-parameter) for antigen and antibody distributions (up to 9 colors with 3 lasers)
  • Determination of cell viability using Propidium Iodide, or PI with Sybr 14, and Dead/Live cell gating
  • Expression of Green Fluorescence Protein (GFP), or ds-Red
  • Detection of apoptosis with TUNEL or Annexin-V
  • Multicolor identification or sorting of bacteria, parasites, neurons, sperm, or RBC’s
  • Quantitative analysis of the number of molecules of a particular antigen found on the surface of a cell (MESF)
  • Cell division studies using antibodies to bromodeoxyuridine (BrdU)
  • DNA and cell cycle analysis, DNA Index for aneuploidy
  • Mutant isolation
  • Plant cell studies
  • Virus infected cells
  • Sterile sorting into multi-well plates, trays, or onto individual microscope slides with automated cloning device
  • One, two, three or four way sterile tube sorting

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