Electron Microscopes

TEM image of a Node of Ranvier, courtesy of Kim Vanderpool and Prof. John Rash.


JEOL JEM-1400 120 keV and JEOL JEM-2000 EXII Transmission Electron Microscopes

The core (transmission) electron microscopy laboratory at Colorado State University is a research and service facility, serving the biological and materials research communities at CSU. The TEM is located in room W13 of the Anatomy/Zoology building. It is open to state, university, and outside users.

We provide imaging and preparation for transmission electron microscopy. Instrument use and technician services are available on a recharge fee basis. Clients can be trained in specimen preparation and to use the instruments themselves or can request assistance from the staff.

The Electron Microscopy Laboratory has equipment for imaging and analyzing specimens by routine methods. The laboratory also houses equipment for embedding and sectioning specimens.

Equipment
JEOL JEM-1400 120kV Transmission Electron Microscope
 is equipped with several different specimen holders (a single specimen holder, ±26°; a high tilt specimen holder, ±70°; a Fischione dual axis Tomography holder, ±70°; and a Gatan liquid nitrogen-cooled specimen holder, ±70°), an Orius fastscan digital camera, and a Gatan Ultrascan high-resolution digital camera.  Direct magnification range on this TEM is between 50x and 1,200,000x.

JEOL JEM-2000EXII Transmission Electron Microscope is equipped with a ±60° tilting (goniometer) stage, operates at accelerating voltages from 80kV to 200kV, and has a magnification range from 50x to 500,000x. This TEM is capable of selected area diffraction from 100 to 2,500mm. Two specimens can be loaded simultaneously. Images are currently captured on film, which is processed daily.

Preparatory equipment:

  • Ultramicrotomes: Riechert Ultracut E, and PorterBlum MT2B
  • Glass knife breaker
  • Various equipment for embedding samples
  • Kinney Carbon evaporator

Billing Rate Plan for Transmission Electron Microscope for CSU Internal Users (rates apply to both microscopes)

The following billing rates are designed to cover the annual costs of facility operation and service. The use of the facility is more costly for minor users because they have less training and require more supervision, so a billing scale that rewards greater use is proposed in which major users can invest in the service contract costs up front. The hourly maintenance fee is charged for every hour used.

Please note that these prices are subject to change pending revision of the business plan for these instruments. As of September 2017, a revised business plan is still under development.

Category

Upfront Buy-In

Hourly Maintenance

Costs for Time

Beam Time

Total Cost/h

Occasional (no contract) NONE $10 Over 0 h $50/h $60
7.5% SC costs $2,000 $10 Over 50 h @ $40/h $50
11% SC costs $3,000 $10 Over 100 h @ $30/h $40
18.6% SC costs $5,000 $10 Over 200 h @ $25/h $35/h
30% SC costs $8,000 $10 Over 400 h @ $20/h $30/h or less

Maximum costs are capped at $15,000 per year for major users. The hourly maintenance costs cover liquid nitrogen and daily supervision amounting to about 4h per week. Each group will provide its own film or be charged cost of the film as an additional charge. The up front buy-in will be charged as a percentage of the service contract, which is due July 1.

Multiple groups who want to share a contract may do so ONLY if they use the same trained operator because costs associated with multiple operators for occasional users are much higher than for the major users.

Fee-for-service costs are $50/h for technical support plus one of the charges for cost/h listed above.

Costs for external clients are $100/hr for beam time/maintenance and $80/hr for technical support.

Faculty Supervisor:

John Rash
Department of Biomedical Sciences
Neurosciences Division
Colorado State University
1617 Campus Delivery
Ft. Collins, CO 80523-1617
phone:: 970-491-5606
fax: 970-491-7907 or -2802
john.rash@colostate.edu

Facility Supervisor:

Kim Vanderpool
Department of Biomedical Sciences
Neurosciences Division
Colorado State University
1617 Campus Delivery
Fort Collins, CO 80523-1617
phone: 970-491-7389
fax: 970-491-7907 or -2808
iseetinycells@gmail.com 

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