IACUC Policies and Guidelines

As a research and teaching institution, Colorado State University is obligated to meet federal standards regarding their animal care policies and practices. These include oversight from the US Dept. of Health and Human Services (HHS) and the US Dept. of Agriculture (USDA).

All personnel using animals for research, testing or teaching are required to comply with applicable federal regulations. CSU IACUC considers the federally-mandated policies provide minimum requirements for ethical care and use of animal subjects, and believes that the ethical values of CSU may require a higher standard of care. Therefore, CSU IACUC has developed a number of Policies and Guidelines that communicate these enhanced expectations regarding animal care and use.

  • Policies – practices to which faculty and staff must adhere in their use of animals for research, testing and teaching at CSU.
  • Guidelines, Plans, Directions, etc. – best practices which should be adhered to in the care and use of animals.

If any member of the campus community has questions or concerns about these policies or guidelines, or animal care in general, please contact us. All concerns are kept confidential, and are protected by Whistleblower statutes.


  1. IACUC

    1. IACUC Composition and Responsibilities Policy

      1. Purpose
      2. IACUC Membership
      3. IACUC Responsibilities
      4. Enforcement of Principles
    2. Policy on the Use of Live Vertebrate Animals

      1. When is IACUC approval required?
      2. Inter-institutional Agreements
      3. When is IACUC approval not required?
  2. Training, Personnel, and Animal Facilities

    1. Policy on Animal Care and Use Program Training

      1. Required for all personnel working with animals
      2. Relevant hands-on procedural and species-specific training
      3. PI documentation requirements
    2. Animal Facilities Manager Responsibilities Policy

      1. Roles and Responsibilities of Managers of CSU Animal Facilities
      2. Facility Operations
      3. Training
      4. Compliance
      5. Veterinary Care Program
    3. Principal Investigator Policy

      1. PI Definition and Responsibilities
      2. CSU PI Eligibility
      3. PIs for Collaborations between CSU and Non-CSU Entities
    4. Policy on Purchasing Animals at CSU

      1. Designated Purchasing Subcodes
      2. Prohibition on use of PCard and Disbursement Vouchers
    5. Investigator Managed Care of Animals
  3. Animals

    1. Policy on the Adoption of Research Animals
    2. Policy on the Use of Non-CSU Owned Animals

      1. Use of Client, Faculty, Staff, and Student-Owned Animals
      2. Use of Shelter-Derived Animals
    3. Policy on Wildlife Management and Nuisance or Invasive Species

      1. Wildlife Management Involving Contact with Wild Animals
      2. Activities that Focus on Nuisance or Invasive Species
      3. Activities that May Incidentally Encounter Nuisance or Invasive Wildlife Species
      4. Cooperative Extension Activities Involving Nuisance and Invasive Wildlife Species
      5. Routine Control of Nuisance or Invasive Species on CSU Properties
      6. General Expectations of CSU Personnel Working with Wildlife
  4. Procedures

    1. Policy on Ascites Production

      1. Scientific Justification
      2. Requirements for Animal Monitoring
    2. Policy on Compounding Pharmaceuticals
    3. Policy on Expiration Dates of Pharmaceuticals in Research Animals

      1. Clear labeling system for pharmaceuticals
      2. Color coded stickers with numbers
    4. Policy on Food and/or Fluid Regulation

      1. Requirement for Scientific Justification
      2. Requirement for Monitoring Animals
      3. Weight Loss Limitation
      4. Restriction in Growing Animals
      5. Coordination with Animal Care Staff
    5. Policy on the Maintenance of Anesthetic Vaporizers

      1. Proper Flow – service machine at least every two years
      2. Machine Maintenance
      3. Personnel Safety
    6. Policy on Multiple Surgical Procedures

      1. Definitions
      2. Requirement for Appropriate Pain Relief
      3. Requirement for IACUC Approval of Multiple Major Survival Surgeries (MMSS)
      4. Requirement of Documenting Surgical and Post-surgical Care
      5. Exception to Prohibition on Multiple Major Survival Surgeries on Unrelated Studies
      6. Multiple Minor Surgical Procedures
    7. Policy on Social Management of Animals

      1. Requirement for Social Housing and Allowable Reasons for Single Housing
      2. IACUC or Veterinary Approval Necessary for Single Housing
    8. Policy on “Tail Biopsy”

      1. Potential Less-Invasive Methods of Tissue Collection for DNA Analysis
      2. Requirement for Analgesia and Post-procedural Care
    9. Policy on the Use of Expired Materials in Research/Testing/Teaching Animals

      1. Prohibition on Use of Expired Anesthetics or Euthanasia Drugs
      2. Other Classes of Drugs
      3. Non-pharmaceutical Supplies and Materials for Survival Procedures
      4. Re-Sterilization of Supplies
    10. Policy on the Use of Non-Pharmaceutical Grade Substances

      1. Requirement for IACUC Review of Non-Pharmaceutical Grade Substance Use
      2. Reporting Animal Welfare Concerns
    11. Animal Transportation Policy

      1. Personal vehicles
      2. Wild animals
      3. Livestock
      4. Building-to-building transport
      5. Animals with Infectious Disease
    12. Observation of Animals in Progressive Disease Studies
  5. Reporting Animal Welfare Concerns

    1. Policy on Reporting and Evaluation of Concerns Regarding Animal Care and Use

      1. Imperative for Reporting Concerns
      2. Methods for Reporting
      3. Confidentiality and Prohibition against Retaliation
      4. Investigation of Reports
      5. Reporting of Findings
    2. Policy on Instances of Noncompliance

      1. Requirement for IACUC Oversight
      2. Reporting Noncompliance
      3. Examples of Instances of Noncompliance
      4. Investigation of Reported Noncompliance
      5. Consultation with IO and Formal Determination of Noncompliance
      6. Corrective Actions that May Arise from Determinations of Noncompliance
      7. Suspension of Animal Research Activities
      8. Reporting Noncompliance to External Agencies

IACUC Guidelines, SOPs, etc.

  1. Protocol Review Process
  2. Performance of Repeat Procedures
  3. Post-Approval Monitoring (PAM) Guidance
  4. Guidelines on Pain Categories
  5. Guidelines for Rodent Survival Surgery

    1. Requirements for Rodent Survival Surgery
    2. Surgery Facilities
    3. Preparation
    4. Postoperative Care
    5. Records
    6. Requirements for Non-survival Rodent Surgeries
    7. Sample Anesthesia and Postoperative Care Record
  6. Guideline on Environmental Enrichment
  7. Guidelines for Euthanasia of Rodents Based on Humane Criteria

    1. General Humane Endpoints
    2. Death as an Endpoint
    3. Tumor Burden
    4. Non-Experimental (Humane) Endpoints
    5. Euthanasia
    6. LAR Rodent Behavioral Scoring
    7. Sample Behavioral Score Sheet
  8. Guidelines on the Acquisition of Livestock Used for Biomedical Research, Testing or Teaching
  9. Directions for CO2 Euthanasia of Rodents
  10. Plan to Provide Exercise for Dogs
  11. CSU IACUC Training Plan
  12. Institutional Statement on Research at CSU
  13. Position Statement in Support of Researchers Utilizing Animal Models

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